Application of Seven Antithrombotic Agents
There are two kinds of proteins with antithrombotic effect in snake venom, one is able to The enzyme connected with fibrinolytic enzyme is called fibrinolytic enzyme (plasmine，FLE)。 In addition to degrading fibrin and fibrinogen,No activation of plasminogen in human body, no hydrolysis of other coagulation factors And platelet membrane, with obvious antithrombotic effect. The other is by suppressing Prothrombin preparation is activated to delay the coagulation process, and has no enzyme activity.
This kind of enzyme was first produced by Atoda et al And discuss the anticoagulation mechanism of the anticoagulant protein, It is confirmed that the effect of coagulation factor IX or coagulation factor X on calcium ion Forming 1:1 complex to prolong the coagulation time; Later, Lu et al. from Agkistrodon acutus in southern Anhui A anticoagulant factor ACFI was isolated from snake venom. Liu Qing Liang et al. found that ACFI contains calcium ion,It was also confirmed that its anticoagulant activity and spatial structure were related to calcium ions.Later, Xu Xiaolong and others separated the venom of Agkistrodon acutus from Agkistrodon acutus by an improved separation method Another anticoagulant factor ACFI I The mechanism implementation study found that it is similar to AC FI and belongs to the second category. Zhai Ning et al. isolated a ne anticoagulant factor from the venom of Agkistrodon acutus, and Its physical and chemical properties were studied, and it was found that it had no fibrinolytic activity and no phospholipase A 2 activity and bleeding activity. Qian Xiaojiang and others separated the venom of Agkistrodon acutus from Hunan The fibrinolytic active component was isolated and confirmed by thrombolytic test in vitro It has obvious thrombolytic effect. Lei Danqing was separated by three chromatography methods And the activity of plasmin is greatly improved. Because the plasmin component contains The bleeding component is difficult to be separated, and its clinical application value is not obvious. Li Zhaoyan et al Through efforts to obtain fibrinolytic enzyme without bleeding components Technical support is provided by. Zhou Xianli [18] proved through experiments that Thrombin like enzyme and plasmin from Agkistrodon halys venom have obvious thrombolytic effect, but The direct effect of plasmin is stronger than that of thrombin like enzyme, Therefore, thrombin like enzyme is a slow thrombolytic agent, which makes the recanalization of blood vessels gradual, Enhance the tolerance of the blood vessels in the ischemic area, and survive the vascular rupture caused by blood perfusion Cleavage has a good effect on the early stage of thrombosis, and both are used together, It can reduce the dosage and achieve anticoagulant and thrombolytic effects.Application of Seven Antithrombotic Agents There are two kinds of proteins with antithrombotic effect in snake venom, one is able to The enzyme connected with fibrinolytic enzyme is called fibrinolytic enzyme (plasmine，FLE)。 In addition to degrading fibrin and fibrinogen,No activation of plasminogen in human body, no hydrolysis of other coagulation factors And platelet membrane, with obvious antithrombotic effect. The other is by suppressing Prothrombin preparation is activated to delay the coagulation process, and has no enzyme activity.
This kind of enzyme was first produced by Atoda et al And discuss the anticoagulation mechanism of the anticoagulant protein, It is confirmed that the effect of coagulation factor IX or coagulation factor X on calcium ion Forming 1:1 complex to prolong the coagulation time; Later, Lu et al. from Agkistrodon acutus in southern Anhui A anticoagulant factor ACFI was isolated from snake venom. Liu Qing Liang et al. found that ACF contains calcium ion It was also confirmed that its anticoagulant activity and spatial structure were related to calcium ions. Later, Xu Xiaolong and others separated the venom of Agkistrodon acutus from Agkistrodon acutus by an improved separation method Another anticoagulant factor ACFI I The mechanism implementation study found that it is similar to AC FI and belongs to the second category. Zhai Ning et al. isolated a new anticoagulant factor from the venom of Agkistrodon acutus, and Its physical and chemical properties were studied, and it was found that it had no fibrinolytic activity and no phospholipase A 2 activity and bleeding activity. Qian Xiaojiang and others separated the venom of Agkistrodon acutus from Hunan The fibrinolytic active component was isolated and confirmed by thrombolytic test in vitro It has obvious thrombolytic effect. Lei Danqing was separated by three chromatography methods And the activity of plasmin is greatly improved. Because the plasmin component contains The bleeding component is difficult to be separated, and its clinical application value is not obvious. Li Zhaoyan et al Through efforts to obtain fibrinolytic enzyme without bleeding components Technical support is provided by. Zhou Xianli [18] proved through experiments that Thrombin like enzyme and plasmin from Agkistrodon halys venom have obvious thrombolytic effect, but The direct effect of plasmin is stronger than that of thrombin like enzyme, Therefore, thrombin like enzyme is a slow thrombolytic agent, which makes the recanalization of blood vessels gradual, Enhance the tolerance of the blood vessels in the ischemic area, and survive the vascular rupture caused by blood perfusion Cleavage has a good effect on the early stage of thrombosis, and both are used together
It can reduce the dosage and achieve anticoagulant and thrombolytic effects.
Post time: Oct-19-2022