Objective To investigate the effect and phase of the component of Agkistrodon acutus venom ACTX-8 on the adhesion and invasion of cervical cancer cell Hela
Shutdown system. Methods Fibronectin was coated in 96-well plate. Hela cells passed through 0, 2.5, 5, 10, 20, 40 g/ml of ACTX-8
After treatment, the adhesion with Fibronectin was detected, and the number of adherent cells was detected by MTY; Detection of cell invasion and migration by TransweU cell method
Changes in capabilities; The competitive binding between ACTX-8 and Fibronectin and Hela cell membrane was detected by flow cytometry. Results:
After treatment with ACTX-8, the adhesion of Hela cells to Fibronectin decreased, and the transwell cell experiment showed that Hela cells migrated
The ability of migration and invasion is also reduced. The flow cytometry results show that ACTX-8 can compete to bind the receptor of Fibronectin on the cell.
Post time: Feb-18-2023