news1

How to treat retinal vein obstruction?

[Abstract] Objective To isolate and purify anticoagulant protein from Agkistrodon acutus venom in southern Anhui and observe its inhibitory effect on human vascular endothelial cells. Methods Human vascular endothelial cells EC-304 were cultured in vitro. CCK-8 colorimetry was used to determine the cytotoxicity of the anticoagulant protein fraction of Agkistrodon halys venom on EC-304 cells cultured in vitro. Phase contrast microscopy was used to observe the changes in cell morphology and biochemistry of EC-304 cells treated with the anticoagulant protein fraction of Agkistrodon halys venom. Results The anticoagulant protein fraction of Agkistrodon halys venom had obvious growth inhibition on EC-304 cells (P μ The anticoagulant protein fraction of Agkistrodon halys venom (g/mL) had a strong inhibitory effect on EC-304 cells, and the inhibition rate increased with the increase of the dose; The EC-304 cells treated with anticoagulant protein from Agkistrodon halys venom showed morphological changes. Under the microscope, the cells were round, the number of suspended cells increased, the number of cell division phases decreased, and the number of cell fragments in the culture medium increased significantly. Conclusion The anticoagulant protein fraction of Agkistrodon acutus venom from southern Anhui Province has a strong inhibitory effect on EC-304 cells, and inducing apoptosis of EC-304 cells may be one of its mechanisms.


Post time: Dec-29-2022