Inhibitory effect of antitumor component I of Agkistrodon halys venom on proliferation of human lung cancer A549 cells

[Abstract] Objective: To study the effect of Agkistrodon acutus venom tumor suppressor component I (AAVC-I) on the proliferation inhibition and apoptosis of human lung cancer A549 cells. Methods: The inhibition rates of AAVC-I at different concentrations on A549 cells for 24h and 48h were measured by MTT method; HE staining and Hoechst 33258 fluorescence staining were used to observe apoptosis from morphology; The expression of bax protein was detected by immunohistochemistry. Results: MTT showed that AAVC-I could inhibit the proliferation of A549 cells in a time-dependent and dose-dependent manner; After AAVCI treatment for 24 hours, nuclear pyknosis, nuclear hyperchromatic and apoptotic bodies were observed under microscope; Immunohistochemistry showed that the average optical density increased with the increase of drug concentration, indicating that the expression of bax protein was correspondingly up-regulated. Conclusion: Antitumor component I of Agkistrodon acutus venom can inhibit human lung cancer A549 cells and induce apoptosis, which may be related to the up-regulation of bax expression.

Post time: Jan-16-2023