[Abstract] Haemocoagulase Acutus (Halase), a new thermostatic in halase development, is a high performance thrombobin like enzyme (TLE) derived from snake venom of Agkistrodon acutus To study the pharmacokinetics of Halase, 125Ⅰ-labeled radioisotope method and radioassay combined with TCA precip-itate were applied in rats. Results showed that AUC values were positively correlated with doses under both used methods,and the correlation coefficients were 0.999 8 and 0.999 0 respectively; drug concen-tration in liver,spleen and heart reached its peak after 5 minutes of administration while most of other or-gans reached it in 30 minutes, and then gradually decreased; in each time point,drug concentration in the liver was significantly higher than that in other tissues; Halase was completely excreted and mainly in urine.% Thrombin from Agkistrodon acutus is a kind of thrombin isolated and purified from the venom of Agkistrodon acutus. It is a new type of hemostatic drug developed by our team The pharmacokinetics of thrombin from Agkistrodon acutus in rats was studied by 125 I-labeled radioisotope method and trichloroacetic acid precipitation combined with radioactive detection. The results showed that the AUC values measured by the two methods were positively correlated with the dose, and the correlation coefficients were 0.9998 and 0.9990, respectively; The drug content in liver, spleen and heart was the highest 5 min after administration, while that in most other tissues was the highest 30 min after administration, and then gradually decreased; At each time point, the drug content in liver tissue was significantly higher than that in other tissues; In addition, the thrombin excretion of Agkistrodon acutus is complete, mainly by urine
Post time: Dec-08-2022