[Abstract] Objective To investigate the inhibitory effect of small molecular polypeptide fraction (K fraction) from Agkistrodon acutus venom on the proliferation of human ovarian cancer cell line A2780 and its mechanism. Methods MTT assay was used to detect the growth inhibition of K component on cancer cell lines; The anti-cell adhesion effect of K component was observed by adhesion test; AO-EB double fluorescence staining and flow cytometry were used to detect the occurrence of apoptosis. Results K component inhibited the proliferation of human ovarian cancer cell line A2780 in a time-effect and dose-effect relationship, and could resist the adhesion of cells to FN. Apoptosis was detected by AO-EB double fluorescence staining and flow cytometry. Conclusion Component K has a significant inhibitory effect on the proliferation of human ovarian cancer cell line A2780 in vitro, and its mechanism may be related to anti-cell adhesion and induction of apoptosis.
Post time: Jan-11-2023