To study the effect of thrombin like enzyme and plasmin isolated from the venom of Agkistrodon acutus on blood coagulation system Methods: Thrombin like enzyme and plasmin were isolated and purified from Agkistrodon acutus venom by DEAE Sepharose CL-6B and Sephadex G-75 chromatography, and their effects on coagulation system were observed through in vivo experiments Results: Thrombin like enzyme and plasmin were isolated from the venom of Agkistrodon acutus, and their relative molecular weights were 39300 and 26600 respectively. In vivo experiments showed that thrombin like enzyme and plasmin from Agkistrodon acutus venom could significantly prolong the whole blood clotting time, activated partial prothrombin time, thrombin time and prothrombin time, and reduce the content of fibrinogen, but the effect of thrombin like enzyme was stronger, However, fibrinolytic enzyme showed anticoagulant effect only at a large dosage, and the combination of the two was better than their single use. Conclusion: Thrombin like enzyme and fibrinolytic enzyme from Agkistrodon acutus venom have a
Post time: Dec-14-2022