IgY antibody against the common motif of metalloproteinases from Agkistrodon acutus venom was prepared and its role in snake venom activity was studied [Methods] By analyzing the common sequences of various metalloproteinases from Agkistrodon acutus venom, we designed and synthesized highly conserved antigenic peptides PT1 and PT2, which are closely related to their enzyme activities; The hens were immunized with the conjugated complexes KLH-PT1 and KLH-PT2 to obtain IgY antibodies. The titer of IgY and the cross reaction characteristics with the venom of Agkistrodon acutus and Agkistrodon brevius were preliminarily studied by ELISA and Western blot; Finally, the neutralizing activity of IgY was evaluated by anti subcutaneous hemorrhage test in mice [Results] ELISA and Western blot showed that anti KLH-PT1 and anti Agkistrodon acutus venom IgY could cross react with Agkistrodon acutus venom and Agkistrodon brevius venom, and the latter was significantly stronger than the former; The anti KLH-PT2 IgY had no significant cross reaction with Agkistrodon acutus and Agkistrodon brevius venoms in vitro, but showed strong neutralizing toxicity in the anti bleeding experiment in vivo, and was significantly superior to the former two [Conclusion] The IgY antibody that can cross react with various blood circulating snake venoms was prepared by designing the antigen peptide shared by metalloproteinases, which laid the foundation for the preparation of specific, efficient, low toxicity and broad-spectrum anti hemorrhagic snake venom antibody
Post time: Dec-26-2022